Journal: PLoS ONE

Article Title: Developing a clinically relevant radiosensitizer for temozolomide-resistant gliomas

doi: 10.1371/journal.pone.0238238

Figure Lengend Snippet: MGMT-expressing variants (i.e., LN229M, U251M, and GL261M) of three well-established GB cell lines were checked along with their parental cells as well as the T98G (an endogenous high MGMT expressor) and LN229TR2 (an MMR-deficient variant of LN229) GB cells for MGMT expression by Western blotting (panel A). Colony survival data are also shown for all three isogenic GB cell lines (panel B). The O6BG MGMT inhibitor (40 μΜ) was used to demonstrate that the artificially expressed MGMT proteins in LN229M, U251M, and GL261M cells were functional and respond to specific inhibition (i.e., the MGMT-positive cells are sensitized to both alkylating agents after the addition of the O6BG inhibitor).

Article Snippet: A rabbit polyclonal antibody that crossreacts with both human MGMT and mouse Mgmt proteins was purchased from Boster Biological Technology (Pleasanton, CA) and used in this Western-blot analysis.

Techniques: Expressing, Variant Assay, Western Blot, Functional Assay, Inhibition

Journal: Structure (London, England : 1993)

Article Title: Structural insights into the assembly and regulation of 2'-O RNA methylation by SARS-CoV-2 nsp16/nsp10.

doi: 10.1016/j.str.2025.03.009

Figure Lengend Snippet: Figure 3. Putative ligand binding pocket of nsp16 (A) Snapshots of various ligands that bind to the pocket in different nsp16/nsp10 structures: adenosine (PDB: 6WKS),8 m7GDP (PDB: 6WQ3),9

Article Snippet: The following antibodies were used: SARS-CoV-2 nsp10 (ProSci, 9179), SARS-CoV-2 nsp16 (ProSci, 9271), anti-rabbit IgG HRP secondary (Cell Signaling, 7074).

Techniques: Ligand Binding Assay

Journal: Structure (London, England : 1993)

Article Title: Structural insights into the assembly and regulation of 2'-O RNA methylation by SARS-CoV-2 nsp16/nsp10.

doi: 10.1016/j.str.2025.03.009

Figure Lengend Snippet: Figure 5. Sampling oligomeric conforma- tions in solution (A) Coomassie staining of crosslinked nsp16/ nsp10 (±RNA) on SDS-PAGE, with protein bands labeled as 1–6. (B) Immunoblotting with nsp16 antibody shows nsp16 in bands 2, 4–6, and part of wider band 3 (3A). (C) Immunoblotting with nsp10 antibody detects nsp10 in bands 1, 5, 6, and parts of wider band 3 (3B and 3C). Cartoon representations of their olig- omeric states are shown below (blue circle: nsp10, green square: nsp16). (D) Coomassie staining of crosslinked quintuple mutant (±RNA), protein bands labeled as 1–6. (E and F) Immunoblotting with nsp16 and nsp10 antibodies, respectively.

Article Snippet: The following antibodies were used: SARS-CoV-2 nsp10 (ProSci, 9179), SARS-CoV-2 nsp16 (ProSci, 9271), anti-rabbit IgG HRP secondary (Cell Signaling, 7074).

Techniques: Sampling, Staining, SDS Page, Labeling, Western Blot, Mutagenesis

Journal: Structure (London, England : 1993)

Article Title: Structural insights into the assembly and regulation of 2'-O RNA methylation by SARS-CoV-2 nsp16/nsp10.

doi: 10.1016/j.str.2025.03.009

Figure Lengend Snippet: Figure 6. Oligomerization via nsp16-nsp16 interfaces and MP analysis (A) nsp16-nsp16 interface 1 in the current struc- ture. (B) Previously reported interface 2 (PDB: 7JYY). (C) An overlay revealing distinct interfaces on opposite faces of the nsp16/nsp10 heterodimer. (D and E) Mass photometry of the (D) WT apo (gray) and RNA-bound (1:1 M ratio, dark green) and (E) quintuple mutant.

Article Snippet: The following antibodies were used: SARS-CoV-2 nsp10 (ProSci, 9179), SARS-CoV-2 nsp16 (ProSci, 9271), anti-rabbit IgG HRP secondary (Cell Signaling, 7074).

Techniques: Mutagenesis